|
KMID : 0829220110350020057
|
|
Korean Journal of Oral and Maxillofacial Pathology
2011 Volume.35 No. 2 p.57 ~ p.65
|
|
|
The Involvement of Heme Oxygenase-1 on Iron Chelator-Induced Osteoblastic Differentiation
|
|
Ham Sun-Do
Lee Sun-Kyung
Kook Yoon-Ah
Kim Young-Il
Shin Kyung-Sub
Lee So-Yun
Bae Won-Jung
Lee Sang-Im
Lee Young-Man
Kang Sun-Il
Park Jae-Kook
Ryu Won-Kook
Chae Jong-Moon
Lee Seoung-Hoon
Kim Eun-Cheol
|
|
|
Abstract
|
|
|
|
The present study aimed to verify the effects of DFO on PDL cells, with particular emphasis on focusing on osteoblastic differentiation. Its mechanisms related to heme oxygenase-1 (HO-1) pathway were also analyzed. DFO increased the expression of HO-1 and early osteoblastic differentiation markers, such as alkaline phosphatase (ALP) and bone sialoprotein (BSP). DFO upregulated heme oxygenase-1. Treatment with HO-1 siRNA blocked the DFO-stimulated osteoblastic differentiation and HO-1 expression. The NF-kB inhibitor pyrrolidine dithiocarbamate, phosphatidylinositol 3-kinase inhibitor Wortmannin, and p38 MAPK inhibitor U0126 blocked the effects of DFO on HO-1 expression and osteoblastic differentiation in PDL cells. Collectively, these data suggest that DFO promotes osteoblastic differentiation and induces the expression of defense protein HO-1 probably via PI3K, p38 MAPK, and NF-kB signalling pathways in PDL cells.
|
|
|
KEYWORD
|
|
|
Ho-1 osteoblastic differentiation, Human periodontal ligament cells, Iron chelator
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|